DDDDK-tagged Protein PURIFICATION GEL with Elution Peptide
Product Code: MBL-3327
Product Group: Protein Extraction, Isolation, and Purification Kits
Supplier: MBL
Code | Size | Price |
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MBL-3327 | Gel 5 mL, Peptide 25 mg | £2,424.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Background:
Isolation of DDDDK tagged protein with protein activity and native conformation from cell culture supernatants and cell lysate under neutral pH condition.
Description:
The ability to isolate and study a purified protein lies at the heart of modern biochemistry.
Researchers in many fields require highly purified, active proteins for studies involving signaling
pathways, enzymology, receptor binding, DNA binding, post-transcriptional modifications, and
much more. Thus, choosing a method of purification is an important aspect in maintaining protein
structure and function.
Recombinant tagged protein purification methods and kits are now widely recognized. The DDDDK
epitope tag (DYKDDDDK) has been widely used as a multi-purpose tag, and anti-DDDDK-tag
antibodies are optimally suited for identifying, detecting, purifying, and monitoring the expression levels
of recombinant DDDDK-tagged proteins.
MBLI?s DDDDK-tagged Protein PURIFICATION GEL with Elution Peptide is designed for the
isolation of DDDDK-tagged protein from cell culture supernatants and cell lysate under neutral pH
condition. Severe conditions such as acidic or alkaline elution denature protein structure.
However, a neutral pH elution can preserve protein activity and native conformation. Therefore,
MBLI has developed the Anti-DDDDK-tag Gel to purify DDDDK-tagged proteins quickly and
efficiently at neutral pH to maintain the activity and conformation of purified proteins. The elution
of DDDDK-tagged proteins from the Gel is achieved by the addition of the DDDDK-tag peptide
(DYKDDDDK). As the DDDDK-tag peptide competes with DDDDK-tagged proteins on the Gel, the
purified proteins do not lose the protein activity.
Kit Components:
Anti-DDDK tag beads, elution peptide, spin column sets, wash concentrate
Shelf Life:
1 year
Target:
DDDDK