IL-33 (mouse) (rec.) (His)

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AdipoGen Life Sciences
Product Code: AG-40A-0053
Product Group: Recombinant Proteins
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AG-40A-0053-C01010 ug£120.00
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AG-40A-0053-C05050 ug£340.00
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Overview

Host Type: E.coli
Regulatory Status: RUO
Target Species: Mouse
Shipping:
Blue Ice
Storage:
-20°C

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Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and
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Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and

Documents

Further Information

Alternate Names/Synonyms:
Interleukin-33; IL-1F11; NF-HEV
Biological Activity:
Activates mouse and human ST2-dependent NF-kappaB pathway.
EClass:
32160000
Endotoxin:
<1EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Liquid. 0.2µm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM NaCl.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Long Description:
Protein. Mouse IL-33 (aa 109-266) is fused at the C-terminus to a His-tag. Source: E. coli. Endotoxin content: <1EU/µg purified protein (LAL test; Lonza). Liquid. 0.2µm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM NaCl. Binds to mouse and human ST2. Purity: >90% (SDS-PAGE). Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.
Molecular Weight:
~20kDa (SDS-PAGE)
NCBI, Uniprot Number:
Q2YEJ4
Package Type:
Plastic Vial
Product Description:
Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.
Purity:
>90% (SDS-PAGE)
Sequence:
Mouse IL-33 (aa 109-266) is fused at the C-terminus to a His-tag.
Source / Host:
E. coli
Specificity:
Binds to mouse and human ST2.
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Interleukins
UNSPSC Number:
41116127
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.