Caspase-10 Antibody
| Code | Size | Price |
|---|
| PSI-1128-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-1128-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Caspase-10 Antibody: MCH4, Caspase-10, Apoptotic protease Mch-4, CASP-10
Application Note:
Caspase-10 antibody can be used for detection of caspase-10 by Western blot 1 μg/mL. A 59 kDa band can be detected.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Caspase-10 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4 and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is therefore involved in the CD95 and TNFR1 induced apoptosis. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8 and -9, which causes the proteolytic cleavage of many key proteins such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase-10 is expressed in many tissues and cell lines.
Background References:
- Vincenz.C. and Dixit,V.M. Fas-associated death domain protein interleukin-1β-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling. J. Biol. Chem. 1997;272:6578-6583
- Fernandes-Alnemri T, Armstrong RC, Krebs J, Srinivasula SM, Wang L Bullrich F, Fritz LC, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc. Natl. Acad. Sci. USA. 1996;93:7464-7469
- Cohen GM. Caspases: the executioners of apoptosis. Biochem J 1997;326:1-16 (RD1299)
Buffer:
Caspase-10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Caspase-10 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Caspase-10.
The immunogen is located within amino acids 240 - 290 of Caspase-10.
The immunogen is located within amino acids 240 - 290 of Caspase-10.
NCBI Gene ID #:
843
NCBI Official Name:
caspase 10, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAD28402
Protein GI Number:
4731237
Purification:
Caspase-10 Antibody is Antibody is DEAE purified.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
Antibody only recognizes FLICE2 form of caspase-10.
Swissprot #:
Q92851
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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