Caspase-9 Antibody

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ProSci
Product Code: PSI-2071
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2071-0.02mg0.02mg£150.00
Quantity:
PSI-2071-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071 (1 μg/mL), Caspase 9, 2073 (1 μg/mL) and beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071 (1 μg/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human Heart</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL Lane 2: 2 μg/mL Lane 3: 4 μg/mL
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<strong>Figure 4 Western Blot Validation in HeLa Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071 (Lane 1: 0.5 μg/mL; Lane 2: 1 μg/mL and Lane 3: 2 μg/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 Western Blot Validation in Mouse Spleen Tissue</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071 (Lane 1: 0.5 μg/mL; Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 μg/mL Lane 2: 1 μg/mL and Lane 3: 2 μg/mL
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<strong>Figure 6 Western Blot Validation in Rat Spleen Tissue</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071 (Lane 1: 0.5 μg/mL; Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071 (1 μg/mL), Caspase 9, 2073 (1 μg/mL) and beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071 (1 μg/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Heart</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL Lane 2: 2 μg/mL Lane 3: 4 μg/mL
<strong>Figure 4 Western Blot Validation in HeLa Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071 (Lane 1: 0.5 μg/mL; Lane 2: 1 μg/mL and Lane 3: 2 μg/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Western Blot Validation in Mouse Spleen Tissue</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071 (Lane 1: 0.5 μg/mL; Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 μg/mL Lane 2: 1 μg/mL and Lane 3: 2 μg/mL
<strong>Figure 6 Western Blot Validation in Rat Spleen Tissue</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Caspase 9, 2071 (Lane 1: 0.5 μg/mL; Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Documents

Further Information

Additional Names:
Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9
Application Note:
WB: 0.5-2 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested.
Background:
Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
Background References:
  • Duan et al. J Biol Chem 1996;271:16720-4.
  • Srinivasula et al. J Biol Chem 1996;271:27099-106.
  • Li et al. Cell 1997;91:479-89.
  • Pan et al. J Biol Chem 1998;273:5841-5 (RD1299).
Buffer:
Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti- Caspase 9 antibody (2071) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human Caspase 9.

The immunogen is located within amino acids 30-80 of Caspase 9.
ISOFORMS:
Human Caspase 9 has 4 isoforms, including isoform 1 (416aa, 46kD), isoform 2 (266aa, 30kD), isoform 3 (154aa, 17kD), and isoform 4 (333aa, 37kD). Mouse Caspase 9 has one isoform (454aa, 50kD) and rat Caspase 9 also has one isoform (454aa, 50kD). 2071 can detect human, mouse and rat.
NCBI Gene ID #:
842
NCBI Official Name:
caspase 9, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46kD

Observed: 46kD
Protein Accession #:
P55211
Protein GI Number:
28558771
Purification:
Caspase-9 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
It has no cross response to other members in caspase family.
Swissprot #:
P55211
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar Caspase 9 expression profile in human cell lines detected by two independent anti-Caspase 9 antibodies that recognize different epitopes, 2071 against N-terminus domain and 2073 against central domain.  Caspase 9 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

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