NOD2 Antibody

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ProSci
Product Code: PSI-2513
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2513-0.02mg0.02mg£150.00
Quantity:
PSI-2513-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of NOD2 in HeLa cell lysate with NOD2 antibody at (A) 2 and (B) 4 μg/mL.
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Immunocytochemistry of NOD2 in HeLa cells with NOD2 antibody at 10 μg/mL.
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Immunofluorescence of NOD2 in HeLa cells with NOD2 antibody at 20 μg/mL.
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Western blot analysis of NOD2 in HeLa cell lysate with NOD2 antibody at (A) 2 and (B) 4 μg/mL.
Immunocytochemistry of NOD2 in HeLa cells with NOD2 antibody at 10 μg/mL.
Immunofluorescence of NOD2 in HeLa cells with NOD2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
NOD2 Antibody: CD, ACUG, BLAU, IBD1, NLRC2, NOD2B, CARD15, CLR16.3, PSORAS1, Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15
Application Note:
NOD2 antibody can be used for detection of Noxa by Western blot at 2 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
NOD2 Antibody: Apaf-1 and NOD1 are members of a new family, which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2. NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-κB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn's disease that is a main type of chronic inflammatory bowel disease.
Background References:
  • Inohara N, Koseki T, del Peso L, et al. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-κB. J. Biol. Chem. 1999; 274:14560-7.
  • Ogura Y, Inohara N, Benito A, et al. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-κB. J. Biol. Chem. 2001; 276:4812-8.
  • Hugot JP, Chamaillard M, et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease. Nature 2001; 411:599-603.
  • Ogura Y, Bonen DK, Inohara N, et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease. Nature 2001; 411:603-6.
Buffer:
NOD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (77%), Mouse: (77%)
Immunogen:
Rabbit NOD2 polyclonal antibody was raised against a synthetic peptide corresponding to 14 amino acids at the carboxy terminus of human NOD2.

The immunogen is located within the last 50 amino acids of NOD2.
NCBI Gene ID #:
64127
NCBI Official Name:
nucleotide-binding oligomerization domain containing 2
NCBI Official Symbol:
NOD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
114 kDa
Protein Accession #:
Q9HC29
Protein GI Number:
20137973
Purification:
NOD2 Antibody is immunoaffinity chromatography purified IgG.
Research Area:
Apoptosis
SPECIFICITY:
NOD2 has no cross-reaction with NOD1.
Swissprot #:
Q9HC29
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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