Bax Antibody

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ProSci
Product Code: PSI-3351
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3351-0.02mg0.02mg£150.00
Quantity:
PSI-3351-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of Bax in HL-60 cell lysates with Bax antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
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Immunocytochemistry staining of HL-60 cells using Bax at 2 μg/mL.
3 / 3
Immunofluorescence of Bax in HL60 cells with Bax antibody at 2 μg/mL.
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Western blot analysis of Bax in HL-60 cell lysates with Bax antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Immunocytochemistry staining of HL-60 cells using Bax at 2 μg/mL.
Immunofluorescence of Bax in HL60 cells with Bax antibody at 2 μg/mL.

Documents

Further Information

Additional Names:
Bax Antibody: BCL2L4, BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4
Application Note:
Bax antibody can be used for detection of Bax by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Bax Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bax, a pro-apoptotic Bcl-2 family member, is a cytosolic protein that changes conformation and translocates to the mitochondria following apoptotic stimuli. It is thought to share significant functional homology with Bak, another pro-apoptotic Bcl-2 family member, as disruption of bax or bak has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking both show decreased apoptotic capability.
Background References:
  • Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
  • Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
  • Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
  • Oltvai ZN, Milliman CL, and Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993; 74:609-19.
Buffer:
Bax Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Mouse: (81%)
Immunogen:
Bax antibody was raised against a peptide corresponding to 16 amino acids near the amino-terminus of human Bax.

The immunogen is located within the first 50 amino acids of Bax.
NCBI Gene ID #:
581
NCBI Official Name:
BCL2-associated X protein
NCBI Official Symbol:
BAX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAA03619
Protein GI Number:
388166
Purification:
Bax Antibody is Ion exchange chromatography purified.
Research Area:
Apoptosis,Autophagy,Cancer
Swissprot #:
Q07812
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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