Caspase-8 Antibody

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ProSci
Product Code: PSI-3473
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3473-0.02mg0.02mg£150.00
Quantity:
PSI-3473-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 6
Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 μg/mL.
3 / 6
Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 μg/mL.
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Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 μg/mL.
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Western blot analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 μg/ml.
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Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 μg/ml.<br><br>Red: Caspase-8 Antibody (3473) <br> Blue: DAPI staining
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Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 μg/mL.
Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 μg/mL.
Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 μg/mL.
Western blot analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 μg/ml.
Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 μg/ml.<br><br>Red: Caspase-8 Antibody (3473) <br> Blue: DAPI staining

Documents

Further Information

Additional Names:
Caspase-8 Antibody: CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, Caspase-8, Apoptotic cysteine protease, CASP-8
Application Note:
Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Caspase-8 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex. The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain; subsequent oligomerization promotes procaspase-8 autoactivation. FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis.
Background References:
  • Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
  • Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
  • Wolf BB and Green DR. Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem. 1999; 274:20049-52.
  • Muzio M, Chinnaiyan AM, Kischkel FC, et al. FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signaling complex. Cell 1996; 85:817-27.
Buffer:
Caspase-8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A.

The immunogen is located within the last 50 amino acids of Caspase-8.
NCBI Gene ID #:
841
NCBI Official Name:
caspase 8, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
NP_001219
Protein GI Number:
15718704
Purification:
Caspase-8 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Neuroscience,Autophagy,Cancer
SPECIFICITY:
Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.
Swissprot #:
Q14790
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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