NALP3 Antibody

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ProSci
Product Code: PSI-5447
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5447-0.02mg0.02mg£150.00
Quantity:
PSI-5447-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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<strong>Figure 1 WB Validation in Human, Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysate. Antibodies: NALP3 5447, 0.5 μg/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 2 Western Blot Validation in Mouse Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: NALP3 5447, 0.5 μg/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 3 Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: NALP3 5447, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 4 Immunofluorescence Validation of NALP3 in Human K562 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 labeling NALP3 with 5447 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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<strong>Figure 5 Immunofluorescence Validation of NALP3 in Human Molt4 Cells </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 labeling NALP3 with 5447 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 6 Immunofluorescence Validation of NALP3 in Mouse Thymus</strong><br> Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using anti-NALP3 antibody (5447) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 7 Immunohistochemistry Validation of NALP3 in Rat Liver</strong><br> Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-NALP3 antibody (5447) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1 WB Validation in Human, Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysate. Antibodies: NALP3 5447, 0.5 μg/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Western Blot Validation in Mouse Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: NALP3 5447, 0.5 μg/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: NALP3 5447, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4 Immunofluorescence Validation of NALP3 in Human K562 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 labeling NALP3 with 5447 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 5 Immunofluorescence Validation of NALP3 in Human Molt4 Cells </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 labeling NALP3 with 5447 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of NALP3 in Mouse Thymus</strong><br> Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using anti-NALP3 antibody (5447) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of NALP3 in Rat Liver</strong><br> Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-NALP3 antibody (5447) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
NALP3 Antibody: AII, AVP, FCU, MWS, FCAS, CIAS1, NALP3, C1orf7, CLR1.1, PYPAF1, AGTAVPRL, NACHT, LRR and PYD domains-containing protein 3, Cold autoinflammatory syndrome 1 protein
Application Note:
NALP3 antibody can be used for detection of NALP3 by Western blot at 1 μg/mL. Antibody can also be used for Immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
NALP3 Antibody: NALP3, a member of Nod-like receptors, has a crucial role in inflammation and immunity and may be a proximal sensor of cellular stress and danger signals. NALP3 forms a caspase-1 activating molecular complex termed the inflammasome. The inflammasome allows activation of IL-1β, the key player of the inflammation and fever. NALP3 gene encodes a pyrin like protein which contains a pyrin domain, a nucleotide binding site (NBS) domain, and a leucine rich repeat (LRR) motif. NALP3 protein interacts with apoptosis associated speck like protein containing a CARD and may function as an inducer of apoptosis and an activator of NF-κB signaling. Defects in NALP3 have been associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID).
Background References:
  • Chamaillard et al. Cell. Microbiol.2003; 5:581-92.
  • O'Connor et al. J. Immunol.2003; 171:6329-33.
  • Kanneganti et al. Nature 2006; 440:233-6.
  • Agostini et al. Immunity 2004; 20:319-25.
  • Sefik et al. Nature 2022 April 28.
Buffer:
NALP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
NALP3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human NALP3.

The immunogen is located within amino acids 120 - 170 of NALP3.
Isoforms:
Human NALP3 has 6 isoforms, including isoform 1 (1036aa, 118.2kD), isoform 2 (922aa, 106kD), isoform 3 (719aa, 83.5kD), isoform 4 (979aa, 111.9kD), isoform 5 (979aa, 112.3kD), and isoform 6 (1016aa, 116kD). Mouse NALP3 has 4 isoforms, including isoform 1 (1033aa, 118.3kD), isoform 2 (976aa, 112.1kD), isoform 3 (976aa, 112kD), and isoform 4 (829aa, 95.7kD). Rat NALP3 has 1 isoform (1035aa, 118.5kD). 5447 can detect NALP3 in human, mouse, and rat.
NCBI Gene ID #:
114548
NCBI Official Name:
NLR family, pyrin domain containing 3
NCBI Official Symbol:
NLRP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 101, 114 kDa

Observed: 110 kDa
Protein Accession #:
NP_899632
Protein GI Number:
34878690
Purification:
NALP3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
Q96P20
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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