TOR Signaling Pathway Detection Set
| Code | Size | Price |
|---|
| PSI-1805-1Set | 1Set | £1,074.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Antibody Clone: See individual datasheets.
Regulatory Status: RUO
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Background:
The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle progression through its ability to integrate signals from nutrient levels and growth factors. TOR regulation is accomplished through a network of various activators and repressors. It is phosphorylated by Akt, whose activity is indirectly inhibited by the lipid phosphatase PTEN. TOR is normally associated with the regulatory proteins RAPTOR, a scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation, and GΒL, which stimulates TOR?s kinase activity towards downstream proteins. It is further regulated by the proteins Rheb, TSC1 and TSC2, which act to modulate TOR activity. The downstream targets of TOR are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs) whose activation leads to increased protein translation and cell growth.
For images please see PDF data sheet
For images please see PDF data sheet
Background References:
- Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.
- Nave BT, Ouwens M, Withers DJ, et al. Mammalian target of rapamycin is a direct target for protein kinase B: Identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation. Biochem. J. 1999; 344:427-31.
- Yonezawa K, Tokunaga C, Oshiro N, et al. Raptor, a binding partner of target of rapamycin. Biochem. Biophys. Res. Commun. 24; 313:437-441.
- Fingar DC and Blenis J. Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression. Oncogene 2004; 23:3151-71.
Buffer:
PBS containing 0.02% sodium azide.
Concentration:
Antibody 1 mg/mL
Immunogen:
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Physical State:
Liquid
Purification:
Antibodies are supplied as affinity chromatography purified IgG.
User Note:
Optimal dilutions for each application to be determined by the researcher.