Alzheimer's Disease B-Amyloid Protein Detection Set

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ProSci
Product Code: PSI-1812
Supplier: ProSci
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PSI-1812-1Set1Set£628.00
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Overview

Antibody Clone: See individual datasheets.
Regulatory Status: RUO
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Documents

Further Information

Application Note:
These polyclonal antibodies can be used for detection of APP, BACE and BACE2 by immunoblot at 1 - 5 μgg/mL. APP and BACE antibodies can detect their respective proteins via immunohistochemistry at 2 - 10 μg/mL.
Background:
Accumulation of the amyloid-β peptide (Aβ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer?s disease. The βamyloid protein precursor (APP) is cleaved by one of two βsecretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for βsecretase to generate the 4 kDa amyloid-β peptide (Aβ), which is deposited in the Alzheimer?s disease patients? brains. BACE was identified by several groups independently and designated β-site APP cleaving enzyme (BACE) . BACE is a transmembrane aspartic protease and co-localizes with APP. BACE2 also cleaves APP at β-site and at a different site within Aβ. BACE2 locates on chromosome 21q22.3, the so-called ?Down critical region?, suggesting that BACE2 and Aβ may also contribute to the pathogenesis of Down syndrome.

For images please see PDF data sheet
Background References:
  • Vassar R, Bennett BD, Babu-Khan S, et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999; 286:735-41.
  • Sinha S, Anderson JP, Barbour R, et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999; 402:537-40.
  • Acquati F, et al. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett 2000;468:59-64
  • Solans A, et al. A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein beta-secretase. Cytogenet Cell Genet 2000;89:177-184.
Buffer:
PBS containing 0.02% sodium azide.
Concentration:
Antibody 1 mg/mL
Immunogen:
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Physical State:
Liquid
Purification:
Antibodies are supplied as affinity chromatography purified IgG.
Specificity:
Both APP antibodies will react with the C99 fragment of APP.
User Note:
Optimal dilutions for each application to be determined by the researcher.