I kappa B Kinase Detection Set

These polyclonal antibodies can be used for detection of IKKα, IKKβ, IKKγ and IKKε by immunoblot at 0.5 - 2 μg/mL, and by immuncytochemistry (IKKα, IKKβ and IKKγ) or immunohistochemistry (IKKε) at 1 - 10 μg/mL, and Immunoflourescence.

Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB kinase (IKK), which phosphorylates IκB and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IκB-α, while IKKβ phosphorylates both IκB-α and IκB-β. Another molecule in the IKK complex termed IKKγ (also known as NEMO) interacts with IKKβ and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation. IKKγ was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation. IKKε is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1. IKKε message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.

For images please see PDF data sheet

PBS containing 0.02% sodium azide.

Antibody 1 mg/mL

Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.

Liquid

Antibodies are supplied as affinity chromatography purified IgG.

Optimal dilutions for each application to be determined by the researcher.