Magnesium Colorimetric Assay Kit (Yyridil Blue-I Method)

Colorimetric

32160000

liquid

Do not freeze.

1 x 25 ml R-1 Chelate color (ready to use) | 1 x 0.5 ml STD Magnesium (Mg) standard 2mg/dL (ready to use)

This MC-Reagent Magnesium Assay Kit is a direct colorimetric assay based on the Xylidyl Blue-I method without deproteinization of the sample. The magnesium determination is based on the reaction of magnesium with Xylidyl Blue-I (as chelator) at alkaline pH, which yields a purple colored complex. The intensity of the color formed is proportional to the calcium concentration in the sample. Absorbance of the Mg2+-complex is measured at 660nm. Features of this Assay: Quick & Easy to use • Species independent • Highly sensitive, stable and suitable for high-throughput testing • No toxic substances • For cell lysates, serum, plasma and wide variety of biological samples

This MC-Reagent Magnesium Assay Kit is a direct colorimetric assay based on the Xylidyl Blue-I method without deproteinization of the sample. The magnesium determination is based on the reaction of magnesium with Xylidyl Blue-I (as chelator) at alkaline pH, which yields a purple colored complex. The intensity of the color formed is proportional to the calcium concentration in the sample. Absorbance of the Mg2+-complex is measured at 660nm. Features of this Assay: Quick & Easy to use • Species independent • Highly sensitive, stable and suitable for high-throughput testing • No toxic substances • For cell lysates, serum, plasma and wide variety of biological samples

Box

Magnesium (Mg2+) is one of the most abundant trace metals in living organisms, contributing to a variety of biological functions including ATP and nucleic acid processing, energy metabolism, and enzymatic function. Magnesium forms complexes with multiple molecules such as phospholipids and ATP. Approximately 69% of magnesium ions are stored in bone. The rest are part of the intermediary metabolism about 70% being present in free form while the other 30% is bound to proteins (especially albumin), citrates, phosphate, and other complex formers. The Mg2+ serum level is kept constant within very narrow limits. Regulation takes place mainly via the kidneys, especially via the ascending loop of Henle. Low serum levels of magnesium have been associated with metabolic syndrome, diabetes mellitus type 2, and hypertension.

0.2 - 3.5mg/dL

Cell Lysate, Plasma, Saliva, Serum, Tissue Supernatant, Urine

Specific to Mg2+.

Non-hazardous

Chemical Reagent Kits

41116104

12 months after the day of manufacturing. See expiry date on ELISA Kit box.