anti-Caspase-8 (mouse) mAb (3B10)
| Code | Size | Price |
|---|
| AG-20T-0138-C100 | 100 ug | £250.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Flow Cytometry
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
-20°C
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like Protease
Concentration:
1mg/ml
EClass:
32160000
Endotoxin:
<0.1EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant mouse caspase-8 (p20 subunit).
Long Description:
Monoclonal Antibody. Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8. Isotype: Rat IgG1kappa. Clone: 3B10. Applications: ELISA, FACS, ICC, IHC, WB. Liquid. In PBS containing 0.02% sodium azide. Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.
NCBI, Uniprot Number:
O89110
Package Type:
Plastic Vial
Product Description:
Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.
Purity:
>95% (SDS-PAGE)
Specificity:
Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.
References
Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes: L.A. O'Reilly, et al.; Cell Death Differ. 11, 724 (2004) | Stimulation of Toll-like receptor 3 and 4 induces interleukin-1 maturation by caspase-8: J. Maelfait, et al.; J. Exp. Med. 205, 1967 (2008) | Impaired lactation in mice expressing dominant-negative FADD in mammary epithelium: M. Shackleton, et al.; Dev. Dyn. 238, 1010 (2009) | Caspase-8 mediates mitochondrial release of pro-apoptotic proteins in a manner independent of its proteolytic activity in apoptosis induced by the protein synthesis inhibitor acetoxycycloheximide in human leukemia Jurkat cells: K. Kadohara, et al.; J. Biol. Chem. 284, 5478 (2009) | Cytotoxic T lymphocyte perforin and Fas ligand working in concert even when Fas ligand lytic action is still not detectable: D. Hassin, et al.; Immunology 133, 190 (2011) | Necroptotic signaling is primed in Mycobacterium tuberculosisinfected macrophages, but its pathophysiological consequence in disease is restricted: M.S. Stutz, et al.; Cell Death Diff. 25, 951 (2018) | IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death: R. Karki, et al.; J. Immunol. 204, 2514 (2020) | COVID-19 cytokines and the hyperactive immune response: Synergism of TNF-alpha and IFN-gamma in triggering inflammation, tissue damage, and death: R. Karki, et al.; bioRxiv (Preprint) (2020) | The nonreceptor tyrosine kinase SYK drives caspase-8/NLRP3 inflammasome-mediated autoinflammatory osteomyelitis: T.K. Dasari, et al.; 295, 3394 (2020) | RIPK1 Distinctly Regulates Yersinia-Induced Inflammatory Cell Death, PANoptosis: E.K. Subbarao Malireddi, et al.; ImmunoHorizons 4, 789 (2020) | Synergism of TNF-a and IFN-g Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARSCoV-2 Infection and Cytokine Shock Syndromes: R. Karki, et al.; Cell 184, 149 (2021) | Hierarchical Cell Death Program Disrupts the Intracellular Niche Required for Burkholderia thailandensis Pathogenesis: D.E. Place, et al.; . mBio 12, e01059 (2021) | DDX3X coordinates host defense against influenza virus by activating the NLRP3 inflammasome and type I interferon response: S. Kesavardhana, et al.; J. Biol. Chem. 296, 100579 (2021)