LysoGlow84
| Code | Size | Price |
|---|
| AG-CMA-1005-M001 | 1 mg | £220.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
4-(Naphthalene-2-yl)-1H-imidazo[4,5-c]pyridine . TFA
Appearance:
Light yellow solid.
CAS:
1882878-94-5 (3xTFA 1782983-52-1)
EClass:
32160000
Form (Short):
solid
Handling Advice:
Avoid freeze/thaw cycles.Protect from light and moisture.Protect from light when in solution.
InChi:
InChI=1S/C16H11N3.C2HF3O2/c1-2-4-12-9-13(6-5-11(12)3-1)15-16-14(7-8-17-15)18-10-19-16;3-2(4,5)1(6)7/h1-10H,(H,18,19);(H,6,7)
InChiKey:
SSYNPCHXJHAMNE-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 1782983-52-1. Formula: C16H12N3 . C2F3O2. MW: 246.3 . 113.0. Synthetic. Cell/membrane permeable, non-toxic, pH-sensitive fluorescent dye (blue-green range) for live imaging of pH alteration in acidic organelles, lysosomes, autophagolysosomes, vesicles, cells, tissue and even small, transparent whole animals without side effects. Useful as intracellular pH indicator with a wide range of fluorescence (pH 3 to pH 13). pH-dependent dual-emission peaks, can be used for ratiometric evaluation of pH (F400/440). No esterases are involved, therefore no toxic by-products occur. Long-term stable; is barely metabolized. Fluorescence co-localizes with LysoTracker® Red DND-99. (LysoTracker® is a registered trademark of Molecular Probes, Inc.)
MDL:
N/A
Molecular Formula:
C16H12N3 . C2F3O2
Molecular Weight:
246.3 . 113.0
Other data:
Fluorescence/Spectral properties: Excitation 310 - 380nm; maximum at ~320nm Emission 400 - 520nm; maximum at 440nm (pH3 to pH6) and 400nm (pH 8 to pH 13) Prepare a 10mM stock solution in MeOH or DMSO:Dissolve 1mg of LysoGlow84 in 278µl methanol, mix gently until the compound is completely dissolved. The solution should show a homogenous yellow color. Use fresh preparations or aliquot and freeze immediately. LysoGlow84 is light sensitive, avoid long exposure to direct light or other bright light sources. Avoid freeze/thaw cycles of solutions.
Package Type:
Vial
Product Description:
Cell/membrane permeable, non-toxic, pH-sensitive fluorescent dye (blue-green range) for live imaging of pH alteration in acidic organelles, lysosomes, autophagolysosomes, vesicles, cells, tissue and even small, transparent whole animals without side effects. Useful as intracellular pH indicator with a wide range of fluorescence (pH 3 to pH 13). pH-dependent dual-emission peaks, can be used for ratiometric evaluation of pH (F400/440). No esterases are involved, therefore no toxic by-products occur. Long-term stable; is barely metabolized. Fluorescence co-localizes with LysoTracker® Red DND-99. (LysoTracker® is a registered trademark of Molecular Probes, Inc.)
Purity:
>98% (NMR)
SMILES:
[O-]C(=O)C(F)(F)F.[NH2+]1C=NC2=C(N=CC=C12)C1=CC2=CC=CC=C2C=C1
Solubility Chemicals:
Soluble in DMSO, methanol or other polar solvents. Poorly soluble in water at pH8 to pH12.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
12171500
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C. Stable for at least 1 year after receipt when stored at +4°C. Stock solutions are stable for at least 3 months when stored at -20°C.
References
The chemically synthesized Ageladine A-derivative LysoGlow84 stains lysosomes in viable mammalian brain cells and specific structures in the marine flatworm Macrostomum lignano: T. Mordhorst, et al.; Mar. Drugs 13, 920 (2015)