Noggin Recombinant Protein
| Code | Size | Price |
|---|
| PSI-40-445-0.005mg | 0.005mg | £300.00 |
Quantity:
| PSI-40-445-0.02mg | 0.02mg | £428.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Storage:
The lyophilized Noggin recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Noggin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exp
Documents
Further Information
Additional Names:
Noggin
Background:
Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. The interplay between TGF-β ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant murine Noggin is a 46.4 kDa disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.
Biological Activity:
Biological activity was determined by its ability to inhibit 5.0 ng/mL of BMP - 4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is 1.0 - 2.0 ng/mL of Noggin.
NCBI Gene ID #:
18121
NCBI Official Name:
noggin
NCBI Official Symbol:
Nog
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
NP_032737.1
Protein GI Number:
7110675
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Recombinant Protein Sequence:
#N/A
Source:
E. coli
Swissprot #:
P97466