CD55 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-117-0.1mg | 0.1mg | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CD55, DAF, CR, CROM, TC
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
CD55 is also?known?as?DAF?or?decay?accelerating?factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. It prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first SCR. It also binds physiologically?generated C3 convertases with its second and third SCRs. Binding results in an accelerated ?decay?, or dissociation of active C3 convertases, thus blocking the development of C? attack complexes on nonforeign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.
Background References:
- Medof ME, et al., 1987, Proc. Natl. Acad. Sci. U.S.A. 84 (7): 2007?11.
- Karnauchow TM, et al., 1996, J. Virol. 70 (8): 5143?52.
- Spiller OB, et al., 2000, J. Infect. Dis. 181 (1): 340?3.
- Williams,?P.?et?al., 2003, J.?Biol.?Chem.?278:10691.
Biological Activity:
Measured?by?its?binding?ability?in?a?functional?ELISA. When rhCD55 is coated at 1μg/ml (100μl/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0. 15?-2. 0μg/ml.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
1604
NCBI Official Name:
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
NCBI Official Symbol:
CD55
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
35.8 kDa
Protein Accession #:
NP_000565.1
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P08174