G-CSF Recombinant Protein
| Code | Size | Price |
|---|
| PSI-91-001-0.05mg | 0.05mg | £838.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7˚C for 2-7 days.
Aliquots of reconstituted samples are stable at -20˚C for 3 months.
Reconstituted protein solution can be stored at 4-7˚C for 2-7 days.
Aliquots of reconstituted samples are stable at -20˚C for 3 months.
Documents
Further Information
Additional Names:
Granulocyte Colony-Stimulating Factor, G-CSF, Pluripoietin, Filgrastim, Lenograstim, CSF3, C17orf33, GCSF
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.
Biological Activity:
ED50 is less than 0.1 ng/ml as calculated by the dose-dependent proliferation of murine NFS-60 indicator cells.
Specific Activity of 6.0 x 107 IU/ mg.
Specific Activity of 6.0 x 107 IU/ mg.
Buffer:
Lyophilized from a 0.2 um filtered solution of 10mM HAc-NaAc, 150mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Fusion Tag:
Tag Free
NCBI Gene ID #:
1440
NCBI Official Name:
colony stimulating factor 3
NCBI Official Symbol:
CSF3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
18.8 kD
Protein Accession #:
P09919-2
Protein GI Number:
117564
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P09919-2