UIBC Colorimetric Assay Kit
| Code | Size | Price |
|---|
| JAI-CBC-800-100tests | 100 tests | £523.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
BLUE ICE
Storage:
+4°C
Further Information
Alternate Names/Synonyms:
Unsaturated Iron Binding Capacity Assay Kit
Detection Type:
Colorimetric
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Do not freeze.
Kit Contains:
1 x 20 ml R-1 Buffer (Iron concentration 80µg/dL) (ready to use) | 1 x 3 ml R-2 Chelate color (ready to use)
Long Description:
This MC-Reagent UIBC Assay Kit is intended for the quantitative determination of unsaturated iron binding capacity (UIBC) in samples by 96-well reader use. The kit utilizes the chromogen bathophenanthroline to bind Fe2+. Fe3+ is then reduced to Fe2+ by a reducing agent. The Fe2+ reacts with bathophenanthroline to form a colored complex which is measured by 96-well reader or spectrophotometer and is proportional to the amount of iron present. Absorbance of the Fe2+-complex is measured at 546nm and 600nm (reference wavelength).EDTA-Plasma samples cannot be used due to interference with the chromogenic system.
Other data:
This MC-Reagent UIBC Assay Kit is intended for the quantitative determination of unsaturated iron binding capacity (UIBC) in samples by 96-well reader use. The kit utilizes the chromogen bathophenanthroline to bind Fe2+. Fe3+ is then reduced to Fe2+ by a reducing agent. The Fe2+ reacts with bathophenanthroline to form a colored complex which is measured by 96-well reader or spectrophotometer and is proportional to the amount of iron present. Absorbance of the Fe2+-complex is measured at 546nm and 600nm (reference wavelength).EDTA-Plasma samples cannot be used due to interference with the chromogenic system.
Package Type:
Box
Product Description:
Iron is an essential element in mammalians and is contained in various enzymes and is involved in oxidation reactions. Iron is essential during transport of oxygen as composition element of hemoglobin or myoglobin. 30% of the transferrin is associated with Fe3+ in blood. Free transferrin is not associated to iron. TIBC (Total iron binding capacity) = UIBC (Unsaturated iron binding capacity) + Serum iron. TIBC levels change in blood disorders, hepatic diseases, tumors and inflammation. UIBC levels are increased in patients with iron deficiency. Decreased levels of UIBC are seen in patients with infectious diseases, nephrotic syndrome and low proteinosis.
Range:
10 - 800µg/dL
Sample Type:
Plasma, Serum
Specificity:
Detection of unsaturated iron binding capacity (UIBC) by measuring a proportional difference between Fe2+ and Fe3+.
Transportation:
Non-hazardous
UNSPSC Category:
Chemical Reagent Kits
UNSPSC Number:
41116104
Use & Stability:
12 months after the day of manufacturing. See expiry date on ELISA Kit box.
References
Biliary excretion of excess iron in mice requires hepatocyte iron import by Slc39a14: M. Prajapati, et al.; J. Biol. Chem. 297, 100835 (2021)
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