UBE2K [UbcH1 E2-25K] (human) (rec.) (His)

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South Bay Bio
Product Code: SBB-CE0022
Product Group: Recombinant Proteins
Supplier: South Bay Bio
CodeSizePrice
SBB-CE0022-C100100 ug£336.00
Quantity:
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Overview

Regulatory Status: RUO
Target Species: Human
Shipping:
Dry Ice
Storage:
-80C

Images

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Thioester Activity Assay: UBE2K forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of excess DTT. The thioester assay also shows di-ubiquitin formation with addition of ATP. This confirms the activity of
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Thioester Activity Assay: UBE2K forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of excess DTT. The thioester assay also shows di-ubiquitin formation with addition of ATP. This confirms the activity of

Documents

Further Information

Alternate Names/Synonyms:
Ubiquitin-conjugating Enzyme E2; E2-25K; Hip2; HYPG; LIG; UBE2K; UBE2K-25K; Huntingtin-interacting Protein 2
Concentration:
Lot dependent.
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol (v/v), 2mM TCEP.
Handling Advice:
Aliquot to avoid freeze/thaw cycles.
Long Description:
Protein. Human UBE2K (aa 1-200)is fused at the N-terminus to a His-tag. Source: E. coli. Formulation: Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol (v/v), 2mM TCEP. Purity: >95% (SDS-PAGE). UBE2K (UbcH1) is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2K via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2K-Ub thioester. UBE2K is capable of synthesizing K48-linked ubiquitin chains and can do so without an E3 ubiquitin ligating enzyme present.UbcH1 catalyzes free K48-linked polyubiquitin chains and is known to interact with huntingtin and TRIM6. Although an E3 ligase is not required for chain formation, free UBE2K synthesized K48 polyubiquitin chains have been shown to interact with the E3 TRIM6, leading to activation of IKKepsilon kinase activity and subsequent antiviral activity.
Molecular Weight:
~22kDa (SDS-PAGE)
NCBI, Uniprot Number:
P61086
Package Type:
Plastic Vial
Product Description:
UBE2K (UbcH1) is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2K via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2K-Ub thioester. UBE2K is capable of synthesizing K48-linked ubiquitin chains and can do so without an E3 ubiquitin ligating enzyme present. UBE2K catalyzes free K48-linked polyubiquitin chains and is known to interact with huntingtin and TRIM6. Although an E3 ligase is not required for chain formation, free UBE2K synthesized K48 polyubiquitin chains have been shown to interact with the E3 TRIM6, leading to activation of IKKepsilon kinase activity and subsequent antiviral activity.
Purity:
>95% (SDS-PAGE)
Sequence:
Human UBE2K (aa 1-200) (Accession Nr. P61086 http://www.ncbi.nlm.nih.gov/protein/P61086 ) is fused at the N-terminus to a His-tag.
Source / Host:
E. coli
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -80°C.