ISG15 (human) (rec.) (Rhodamine 110)
| Code | Size | Price |
|---|
| SBB-PS0002-C050 | 50 ug | £415.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Shipping:
Dry Ice
Storage:
-80C
Further Information
Alternate Names/Synonyms:
ISG15 Ubiquitin-like Modifier; G1P2; IFI15; IP17; UCRP
Concentration:
Lot dependent.
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM MES pH 6.0, 100mM sodium chloride, 10% glycerol.
Handling Advice:
Aliquot to avoid freeze/thaw cycles.Protect from light.
Labels - Conjugates:
Rhodamine
Long Description:
Protein. Human ISG15 (aa1-157)conjugated at the C-terminus to a quenched Rhodamine 110 dye. Source: E. coli. Formulation: Liquid. In 50mM MES pH 6.0, 100mM sodium chloride, 10% glycerol. Purity: >97% (LCMS). ISG15 (Interferon Stimulated Gene, 15kDa) is a ubiquitin-like modifier which initiates innate immune response by activating RIG-I signaling, stimulating NK-cell proliferation, inhibiting viral budding and acting as an IFNgamma-inducing cytokine. ISG15 contains two tandem ubiquitin homology domains and is cross-reactive with alpha-Ubiquitin antibodies. Conjugation of ISG15 to a substrate protein occurs through a ubiquitin like cascade via an E1 (UBE1L), E2 (UbcH8/UBE2L6) and E3 (not yet discovered.) Deconjugation normally occurs via UBP43 (USP18), however there are several viral proteases that are able to hydrolyze ISG15 conjugates in order to evade immune response. These include the OTU-containing protease of Crimean-Congo Hemorrhagic Fever nairovirus and the Papain-Like Protease (PLPro) of the SARS coronavirus.
Molecular Weight:
~18kDa
NCBI, Uniprot Number:
P05161
Package Type:
Plastic Vial
Product Description:
ISG15 (Interferon Stimulated Gene, 15kDa) is a ubiquitin-like modifier which initiates innate immune response by activating RIG-I signaling, stimulating NK-cell proliferation, inhibiting viral budding and acting as an IFNgamma-inducing cytokine. ISG15 contains two tandem ubiquitin homology domains and is cross-reactive with alpha-Ubiquitin antibodies. Conjugation of ISG15 to a substrate protein occurs through a ubiquitin like cascade via an E1 (UBE1L), E2 (UbcH8/UBE2L6) and E3 (not yet discovered.) Deconjugation normally occurs via UBP43 (USP18), however there are several viral proteases that are able to hydrolyze ISG15 conjugates in order to evade immune response. These include the OTU-containing protease of Crimean-Congo Hemorrhagic Fever nairovirus and the Papain-Like Protease (PLPro) of the SARS coronavirus.
Purity:
>97% (LCMS)
Sequence:
Human ISG15 (aa1-157) (Accession Nr. P05161 http://www.ncbi.nlm.nih.gov/protein/P05161 ) conjugated at the C-terminus to a quenched Rhodamine 110 dye.
Source / Host:
E. coli
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -80°C.