Suc-Leu-Leu-Val-Tyr-AMC [Suc-LLVY-AMC]
| Code | Size | Price |
|---|
| SBB-PS0010-M002 | 2 mg | £101.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Shipping:
Blue Ice
Storage:
4C
Documents
Further Information
Alternate Names/Synonyms:
Proteasome Substrate
Appearance:
Lyophilized powder.
CAS:
94367-21-2
EClass:
32160000
Form (Short):
solid
Handling Advice:
Avoid freeze/thaw cycles.Protect from light.
InChi:
InChI=1S/C40H53N5O10/c1-21(2)16-29(42-33(47)14-15-34(48)49)38(52)43-30(17-22(3)4)39(53)45-36(23(5)6)40(54)44-31(19-25-8-11-27(46)12-9-25)37(51)41-26-10-13-28-24(7)18-35(50)55-32(28)20-26/h8-13,18,20-23,29-31,36,46H,14-17,19H2,1-7H3,(H,41,51)(H,42,47)(H,43,52)(H,44,54)(H,45,53)(H,48,49)/t29-,30-,31-,36-/m1/s1
InChiKey:
UVFAEQZFLBGVRM-FEIGSSFXSA-N
Long Description:
Chemical. CAS: 94367-21-2. Formula: C40H53N5O10. MW: 763.9. Synthetic. Suc-LLVY-AMC is a fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S and 26S proteasome, calpains and other chymotrypsin-like proteases. Cleavage of the peptide by the enzymes liberates the fluorophore AMC which generates strong increase in fluorescence fluorescent that can be monitored fluorimetrically. Excitation=345nm Emission=445nm.
Molecular Formula:
C40H53N5O10
Molecular Weight:
763.9
Package Type:
Plastic Vial
Product Description:
Suc-LLVY-AMC is a fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S and 26S proteasome, calpains and other chymotrypsin-like proteases. Cleavage of the peptide by the enzymes liberates the fluorophore AMC which generates strong increase in fluorescence fluorescent that can be monitored fluorimetrically. Excitation=345nm Emission=445nm.
Purity:
>95% (HPLC)
Sequence:
N-Succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
Solubility Chemicals:
Soluble in DMSO.
Transportation:
Non-hazardous
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.
References
Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates: T. Sasaki, et al.; J. Biol. Chem. 259, 12489 (1984) | Kinetic characterization of the chymotryptic activity of the 20S proteasome: R.L. Stein, et al.; Biochemistry 35, 3899 (1996) | 26S proteasome activity is down-regulated in lung cancer stem-like cells propagated in vitro: J. Pan, et al.; PLoS One 5, e13298 (2010) | Low cell density induced epithelial-mesenchymal transition in MCF10A cell is regulated by proteasome activity: J. Fu, et al.; J. Oncobiomark. 1, 4 (2013) | Marchantin M: a novel inhibitor of proteasome induces autophagic cell death in prostate cancer cells: H. Jiang, et al.; Cell Death Dis. 4, e761 (2013)