E-Selectin Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-868-0.1mg | 0.1mg | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
SELE,RP1-117P20.2,CD62E,ELAM1,ESEL,LECAM2,E-Selectin
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
E-Selectin is also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein.[1] It is transiently expressed on vascular endothelial cells in response to IL-1beta and TNFalpha. E selectin has a cassette structure: an N-terminal, C-type lectin domain, an EGF (epidermal-growth-factor)-like domain, 6 Sushi domain (SCR repeat) units, a transmembrane domain (TM) and an intracellular cytoplasmic tail (cyto).[2] During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. The local release of cytokines IL-1 and TNF-alpha by damaged cells induces the over-expression of E-selectin on endothelial cells of nearby blood vessels.[3] E-selectin mediates the adhesion of tumor cells to endothelial cells, by binding to E-selectin ligands expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, natural killer cells or cancer cells. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.
Background References:
- Collins T, et al., 1991, J. Biol. Chem. 266 (4): 2466?73.
- Graves BJ, et al., 1994, Nature 367 (6463): 532?8.
- Robbins SL, et al., 1999, Philadelphia: WB Saunders. ISBN 0-7216-7335-X.
Buffer:
50 mM Tris, 100 mM Glycine, pH7.5
Fusion Tag:
Fc Tag
NCBI Gene ID #:
6401
NCBI Official Name:
E-selectin precursor
NCBI Official Symbol:
E-Selectin
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
84.8 kDa
Protein Accession #:
NP_000441.2
Purity:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P16581