PD-L1 Antibody [F6A9]

ProSci
Product Code: PSI-SD8639
Product Group: Primary Antibodies
Supplier: ProSci

CodeSizePrice
PSI-SD8639-0.02mg0.02mg£166.00
Quantity:
PSI-SD8639-0.1mg0.1mg£531.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Llama
Antibody Isotype: sdAb
Antibody Clonality: Monoclonal
Antibody Clone: F6A9
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
Dry Ice
Storage:
PD-L1 antibody should be stored in working aliquots at -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Flow cytometry analysis of PD-L1 transfected 293 cells using PD-L1 [F6A9] antibody at 10 μg/ml. Blue: untransfected cells, Yellow: PD-L1 transfected cells.
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Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 μg/ml.

Flow cytometry analysis of PD-L1 transfected 293 cells using PD-L1 [F6A9] antibody at 10 μg/ml. Blue: untransfected cells, Yellow: PD-L1 transfected cells.
Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 μg/ml.

Further Information

Additional Names:
PD-L1 Antibody: B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, B7 homolog 1
Application Note:
PD-L1 antibody can be used for detection of PD-L1 by Flow Cytometry and Immunocytochemistry at 10 μg/ml.
Background:
PD-L1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts.
Background References:
  • Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
  • Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
  • LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
Buffer:
PD-L1 Antibody is supplied in PBS.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PD-L1 antibody was raised against a recombinant protein corresponding to amino acids 19 - 239 of human PD-L1.
NCBI Gene ID #:
29126
NCBI Official Name:
CD274
NCBI Official Symbol:
CD274
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_054862
Protein GI Number:
7661534
Purification:
PD-L1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.??It also contains a myc-tag for detection.
Research Area:
Immunology,Cancer,Cell Cycle
SPECIFICITY:
At least three isoforms of PD-L1 are known to exist.
Swissprot #:
Q9NZQ7
User NOte:
Optimal dilutions for each application to be determined by the researcher.