5-Lipoxygenase Antibody

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NSJ Bioreagents
Product Code: NSJ-R30728
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30728-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the 5-Lipoxygenase antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 5-Lipoxygenase antibody and Lane 1: SW620; 2: Jurkat; 3: COLO320; 4: A549; 5: MCF-7 cell lysate. Predicted/observed size ~78KD
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Western blot testing of 5-Lipoxygenase antibody and Lane 1: SW620; 2: Jurkat; 3: COLO320; 4: A549; 5: MCF-7 cell lysate. Predicted/observed size ~78KD

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the 5-Lipoxygenase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
5-Lipoxygenase is an enzyme that in humans is encoded by the ALOX5 gene. It is a member of the lipoxygenase family of enzymes which also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases. The enzyme 5-lipoxygenase catalyzes 2 reactions in the formation of leukotrienes. Human ALOX5 contains three nuclear localization sequences (NLSs) and a phosphorylation site involved in nuclear localization. Compared with age-matched competent mice, the knockout mice developed less right heart hypertrophy. Pharmacologic inhibition or gene disruption resulted in a significant decrease of beta-amyloid production and gamma-secretase levels. ALOX5 activity is short-lived, apparently in part because of an intrinsic instability of the enzyme.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human ALOX5 (AERNKKKQLPYYYLSPDR) was used as the immunogen for this 5-Lipoxygenase antibody.
Limitation:
This 5-Lipoxygenase antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P09917