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Nordic MuBio

Welcome to Nordic-MUBio - distributed in the UK by Caltag Medsystems

Nordic-MUbio manufacture high quality antibody reagents according to strict ISO 9001 quality guidelines. In December 2013, Nordic-MUbio acquired An Der Grub Bio Research GmbH (ADG), extending their product range with Flow Cytometry products including the ‘gold standard’ FIX&PERM® Cell Permeabilization Kit.

FIX and PERM

FIX&PERM® Cell Fixation and Permeabilization Kit

Intracellular staining of protein biomarkers using antibodies in flow cytometry has opened up a diversity of new options for phenotypic and functional characterisation of cells in both research and clinical diagnosis.

Indeed, definitive phenotypic identification of certain cell types can require labelling of both cell surface and intracellular markers. Additionally, the cytoplasmic localisation of typical membrane molecules can prove to be a most reliable lineage marker, such as cytoplasmic CD3 and CD22 in undifferentiated leukaemia.

In such cases, and for many other cell biological and functional investigations, intracellular immunostaining is a necessity. For this purpose Nordic-MUbio offers a class leading cell fixation and permeabilization kit – FIX&PERM® – which offers a number of advantages over other methods for intracellular staining of cells in flow cytometry.

FIX&PERM®

  • Mildly fixes cells, preserving their flow cytometric scatter characteristics
  • Allows simultaneous characterisation of both intracellular and cell surface markers
  • Rapid technique – whole procedure can be carried out in less than one hour, ready for immediate analysis or storage for 24 hours
  • Stringent QC procedures – the quality of each lot is determined using well-defined blood samples and subsequent comparison of scatter characteristics of obtained leukocyte populations, ensuring consistent and reliable results lot after lot
  • A range of intracellular antibodies with optimised protocols for use with FIX&PERM®

FIX&PERM® is a simple procedure making use of two reagents. Reagent A gently fixes cells, while Reagent B permeabilizes them. The specific formulations reduce background and allow simultaneous addition of permeabilization medium and fluorochrome labelled antibodies, allowing staining of intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins, etc.

FIX&PERM® Reagents

FIX&PERM Sample Kit (CE), (2 x 5ml), Product Code: GAS-002M

FIX&PERM Kit (CE), (2 x 20ml), Product Code: GAS-002

FIX&PERM Kit 1000 (CE), (2 x 100ml), Product Code: GAS-002-1

FIX&PERM Solution A (Fix) (CE), (1 x 100ml), Product Code: GAS-002A-1

FIX&PERM Solution B (Perm) (CE), (1 x 100ml), product Code: GAS-002B-1

FIX&PERM Test Kit, (2 x 1.5 ml), Product Code: GAS-002FOC

Products optimised for use with FIX&PERM®

The majority of available monoclonal antibody conjugates can be used with FIX&PERM®. However, in some cases the fixation step may have to be optimised.

Nordic-MUbio offers a number of mouse anti-human conjugated (PE/FITC) monoclonal antibodies optimised for use with FIX&PERM®.

AntigenCloneIsotypeFormatQuantityCat. No.
CD3UCHT1IgG1Purified0.2 mgGM-4011
   FITC-labeled100 testsGM-4012
   PE-labeled100 testsGM-4013
CD22RFB4IgG1Purified0.2 mgGM-4051
   FITC-labeled100 testsGM-4052
   PE-labeled100 testsGM-4053
Lactoferrin4C5IgG1Purified0.2 mgGM-4111
   PE-labeled100 testsGM-4113
Myeloperoxidase8E6IgG1Purified0.2 mgGM-4191
(MPO-C2)  FITC-labeled100 testsGM-4192
   PE-labeled100 testsGM-4193
LysozymeLZ-2IgG1Purified0.2 mgGM-4131
   FITC-labeled100 testsGM-4132
CD68Ki-M7IgG1FITC-labeled100 testsGM-4152
IL-1 betaFIB3IgG1FITC-labeled100 testsGM-4162
IFN-gammaGZ4IgG1FITC-labeled100 testsGM-4172

Furthermore, a number of Combi-panels are offered for dual immunostaining protocols.

AntigenCloneIsotypeFormatQuantityCat. No.
Negative control Vl-APIgG1FITC-labeled50 testsGIC-201
  Vl-APIgG1PE-labeled  
LysozymeLZ-2IgG1FITC-labeled50 testsGIC-206
Lactoferrin4C5IgG1PE-labeled  
Myeloperoxidase8E6IgG1FITC-labeled50 testsGIC-212
Lactoferrin4C5IgG1PE-labeled  
Myeloperoxidase8E6IgG1FITC-labeled50 testsGIC-213
CD3UCHT1IgG1PE-labeled  
Myeloperoxidase8E6IgG1FITC-labeled50 testsGIC-214
CD22RFB4IgG1PE-labeled  

If you are having difficulty downloading product data or require further information, please contact us.

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