ProSci

Factor XIIIa Antibody [SPM180]

Product Code:
 
PSI-33-778
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM180
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC testing of FFPE human placenta with Factor XIIIa antibody (clone SPM180). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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IHC testing of FFPE human placenta with Factor XIIIa antibody (clone SPM180). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

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PSI-33-778-100ug100ug£765.00
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Further Information

Additional Names:
F13A1, coagulation factor XIII, A1 polypeptide, RP11-232H4.1, F13A, Coagulation factor XIII A chain, Coagulation factor XIII, A polypeptide, TGase, bA525O21.1 (coagulation factor XIII, A1 polypeptide), coagulation factor XIII A1 subunit, factor XIIIa
Application Note:
ELISA: 2-4 ug/ml; order the BSA-free formulation for coating

IF: 1-2 ug/ml

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

WB: 0.5-1 ug/ml

IHC (FFPE): 1-2 ug/ml for 30 min at RT

Titering of the Factor XIIIa antibody may be required for optimal performance.
Background:
Factor XIIIa has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-Factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases are positive), dermatofibrosarcoma protuberans (-/+) and desmoplastic malignant melanoma (-). Anti-Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human partial recombinant protein corresponding to amino acids 46-181 was used as the immunogen for the Factor XIIIa antibody.
NCBI Gene ID #:
2162
NCBI Official Name:
coagulation factor XIII, A1 polypeptide
NCBI Official Symbol:
F13A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
Swissprot #:
P00488
User NOte:
Optimal dilutions for each application to be determined by the researcher