ProSci

Involucrin Antibody [INVO49]

Product Code:
 
PSI-33-944
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
INVO49
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC testing of FFPE human tonsil with Involucrin antibody (clone INVO49)
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IHC testing of FFPE human tonsil with Involucrin antibody (clone INVO49)

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PSI-33-944-100ug100ug£765.00
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Further Information

Additional Names:
IVL,
Application Note:
Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 0.1-0.2 ug/ml for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the Involucrin antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37oC. Enzyme digestion is more effective than Citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Involucrin is a highly reactive, soluble, transglutaminase substrate protein present in keratinocytes of epidermis and other stratified squamous epithelia. It first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase thus helping in the formation of an insoluble envelope beneath the plasma membrane functioning as a glutamyl donor during assembly of the cornified envelope. [Wiki]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Purified protein was used as the immunogen for the Involucrin antibody.
NCBI Gene ID #:
3713
NCBI Official Name:
involucrin
NCBI Official Symbol:
IVL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer, Signal Transduction
Swissprot #:
P07476
User NOte:
Optimal dilutions for each application to be determined by the researcher