ProSci

BrdU Antibody [BRD469]

Product Code:
 
PSI-33-237
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BRD469
Regulatory Status:
 
RUO
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD469).
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IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD469).

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PSI-33-237-100ug100ug£765.00
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Further Information

Application Note:
Flow Cytometry: 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine antibody to be titered up or down for optimal performance.

1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.

Background:
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Bromodeoxyuridine conjugated to KLH was used as the immunogen.
NCBI Gene ID #:
Not Applicable
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cell Cycle
User NOte:
Optimal dilutions for each application to be determined by the researcher