ProSci

Cytokeratin 5/8 Antibody [SPM268]

Product Code:
 
PSI-33-521
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM268
Regulatory Status:
 
RUO
Target Species:
  • Bovine (Cattle)
  • Ferret
  • Hamster
  • Human
  • Mouse
  • Porcine (pig)
  • Rat
  • Sheep
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (SPM268).
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Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (SPM268).

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PSI-33-521-100ug100ug£765.00
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Further Information

Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Western blot: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.

1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at RT
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.
NCBI Gene ID #:
3852, 3856
NCBI Official Name:
keratin 5, keratin 8
NCBI Official Symbol:
KRT5, KRT8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
SPECIFICITY:
Does not react with Chicken & Xenopus.
Swissprot #:
P13647, P05787
User NOte:
Optimal dilutions for each application to be determined by the researcher