Further Information
NEFL, 68 kDa neurofilament protein, CMT1F, CMT2E, NF68, NF-L, NFL, Neurofilament subunit NF-L
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Neurofilament antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. [RefSeq]
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
0.2 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Recombinant human neurofilament light chain protein was used as the immunogen for the Neurofilament antibody.
4747
neurofilament, light polypeptide
NEFL
Homo sapiens
Liquid
Protein G affinity chromatography
Neuroscience, Signal Transduction, Stem Cell
P07196
Optimal dilutions for each application to be determined by the researcher
