ProSci

Recombinant NKX2.2 Antibody [RMNK2-1]

Product Code:
 
PSI-34-046
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG, k
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RMNK2-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC testing of FFPE human pancreas with NKX2.2 antibody.

IHC testing of FFPE human pancreas with NKX2.2 antibody.

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PSI-34-046-100ug100ug£765.00
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Further Information

Additional Names:
NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B
Application Note:
IF: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Titering of the NKX2.2 antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Background:
NKX2.2 acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity). [UniProt]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human partial recombinant protein was used as the immunogen for this rabbit monoclonal NKX2.2 antibody.
NCBI Gene ID #:
4821
NCBI Official Name:
NK2 homeobox 2
NCBI Official Symbol:
NKX2-2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Stem Cell
Swissprot #:
O95096
User NOte:
Optimal dilutions for each application to be determined by the researcher