Progesterone Receptor Antibody [SPM566]

Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, PGR, NR3C3

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the anti-Progesterone Receptor antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This mAb is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Recombinant human protein was used as the immunogen for this anti-Progesterone Receptor antibody.

5241

progesterone receptor

PGR

Homo sapiens

Liquid

Protein G affinity chromatography

Cancer,Neuroscience,Signal Transduction

P06401

Optimal dilutions for each application to be determined by the researcher