Nordic MuBio

Mouse anti Human CD7 conjugated with FITC

Product Code:
 
EXA-0072
Product Group:
 
Primary Antibodies
Supplier:
 
Nordic MuBio
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MG34
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Flow Cytometry
Shipping:
 
Room Temperature
Storage:
 
Product should be stored at 4-8°C. DO NOT FREEZE
 

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EXA-0072100 Tests£221.00
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  • Further Information
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Further Information

Applications Description:
PBMC: Add10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8? C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature 20?C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Background:
Identification of human T lymphocytes in multiple stages of T cell development, including a major subset of mature peripheral T cells. CD7 antigen is often increased on T leukemic cells. The CD7 molecule is a 40,000 M.W. surface antigen that is expressed on T-Lymphoid and myeloid precursors in fetal liver and bone marrow.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Titered for flow cytometry
Field of Interest:
Flow Cytometry
Formulation:
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Functional Analysis:
Flow Cytometry Staining
Immunogen:
CD7=Derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with T-acute lymphoblastic leukemia (T-ALL) cells.
Product Form:
FITC
Product Stability:
Reagents are stable for the period shown on the vial label when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
T-cell antigen CD7; GP40; T-cell leukemia antigen; TP41; Leu-9
UniProt:
P09564 (Human)

References

1. Isolation and characterization of the genomic human CD7 gene: structural similarity with the murine Thy-1 gene. Schanberg, L.E., Fleener, D.E., Kurtzberg, J., Haynes, B.F., Kaufman, R.E.; Proc. Natl. Acad. Sci. USA , 1991 Jan 1;88(2):603-7.

2. Identification of novel B-lineage cells in human fetal bone marrow that coexpress CD7. Grumayer, E.R., Griesinger, F., Hummell, D.S., Brunning, R.D., Kersey, J.H.; Blood, 1991 Jan ; 77(1):64-8.

3. Genuine CD7 expression in acute leukemic and lymphoblastic lymphoma. Osada, H., Emi, N., Ueda, R., Seto, M., Koike, K., Suchi, T., Kojima, S., Obata, Y., Takahashi, T.; Leuk. Res. 1990;14(10):869-77.

4. Inhibition of alloresponsive naive and memory T cells by CD7 and CD25 antibodies and by cyclosporine. Akbar, A.N., Amlot, P.L., Ivory,K., Timms, A., Janossy, G.; Transplantation, 1990 No;50(5):823-9.

5. Comparsion of outcome, clinical, laboratory, and immunological features in 164 children and adults with T-ALL. Garand, R., Vannier, J.P., Bene, M.C., Faure, G., Favre, M., Bernard, A,. Leukemia,1990 No;4(11):739-44.