Rabbit anti Rat Calcium Channel

Product Code:

EXA-X2391P

Product Group:

Primary Antibodies

Supplier:

Nordic MuBio

Host Type:

Rabbit

Antibody Isotype:

IgG

Antibody Clonality:

Polyclonal

Regulatory Status:

RUO

Target Species:

Human
Rat

Applications:

Immunohistochemistry (IHC)
Western Blot (WB)

Shipping:

Ship on gel ice store at -20°C immediately upon arrival

Storage:

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

No additional charges, what you see is what you pay! *

Code	Size	Price

EXA-X2391P

100 ug

£316.00

Quantity:

Prices exclude any Taxes / VAT

How to order with us Contact us

Further Information

Applications Description:

Antibody can be used for Western blotting (1:400 starting dilution). Optimal concentration should be evaluated by serial dilutions.

Background:

Voltage-sensitive calcium channels (VSCCs) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1a gives rise to p and/or q-type calcium currents. P/q-type calcium channels belong to the 'high-voltage activated' (hva) group and are blocked by the funnel toxin (ftx) and by the omega-agatoxin-IVA (omega-aga-IVA). They are however insensitive to dihydropyridines (dhp), and omega- conotoxin-GVIA (omega-ctx-GVIA). voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity.

Caution:

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Concentration:

Lot specific, see vial

Field of Interest:

Signal Transduction

Formulation:

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis:

Western Blotting

Immunogen:

Synthetic peptide derived from the rat 1A calcium channel conjugated to KLH

Positive Control:

Rat brain lysate, human brain tissue

Product Form:

Affinity Purified

Product Stability:

Products are stable for one year from purchase when stored properly

Purification Method:

Antigen Immunoaffiinity Purification

Synonyms:

CaV2.1; P/Q-type Voltage-Gated Ca2+ Channel; Cacna1

UniProt:

P54282 (Rat)

References

1. Starr,T.V.B et.al 'Primary structure of a calcium channel that is highly expressed in the rat cerebellum' Proc. Natl. Acad. Sci. U.S.A. 88, 5621-5625 (1991)

2. Snutch,T.Pet.al 'Rat brain expresses a heterogeneous family of calcium channels' Proc. Natl. Acad. Sci. U.S.A. 87 (9), 3391-3395 (1990)

3. Yu,A.S et.al. 'Molecular characterization and nephron distribution of a family of transcripts encoding the pore-forming subunit of Ca2+ channels in the kidney' Proc. Natl. Acad. Sci. U.S.A. 89 (21), 10494-10498 (1992)

4. Hansen PB, , et al.'Vascular smooth muscle cells express the alpha(1A) subunit of a P-/Q-type voltage-dependent Ca(2+)Channel, and It is functionally important in renal afferent arterioles.' Circ Res (United States) 87(10) p896-902 (2000)

5. Stephens,G.I. et.al 'The Cav2.1/alpha1A (P/Q-type) voltage-dependent calcium channel mediates inhibitory neurotransmission onto mouse cerebellar Purkinje cells.' Eur J Neurosci 13(10) 1902-12 (2001)