Nordic MuBio

Mouse anti Human SNAP

Product Code:
 
EXA-X1026
Product Group:
 
Primary Antibodies
Supplier:
 
Nordic MuBio
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
15D4
Regulatory Status:
 
RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Rat
Applications:
  • Enzyme Immunoassay (EIA)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Room Temperature
Storage:
 
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
 

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CodeSizePrice
EXA-X1026100 ug£316.00
Quantity:
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Further Information

Applications Description:
Optimal concentration should be evaluated by serial dilutions. Clone 4E4 anti-?SNAP specifically recognizes ?-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and does not recognize ?-SNAP

Application: A dilution of 0.5-5 ug/ml is suggested for western blot and 2-10 ug/ml for immunoprecipitation.
Background:
SNAPs (soluble NSF attachment proteins),acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicleto-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. -SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas -SNAP is expressed in all mammalian tissues, -SNAP is expressed only in brain.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol. No preservatives have been included in the antibody solution.
Functional Analysis:
Western Blotting
Immunogen:
Balb/C mice were immunized with recombinant human -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by western blot. The antibody was purified to >95% homogeneity by standard chromatographic techniques
Product:
100 ug monoclonal antibody in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol. No preservatives have been added.
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
The antibody was purified to >95% homogeneity by standard chromatographic techniques
Source:
Monoclonal antibody 15D4 obtained immunization of Balb/C mice with recombinant human alpha -SNAP
Specificity:
Human alpha -SNAP
Synonyms:
alpha/beta SNAP, mouse anti alpha soluble NSF attachment protein,SNAPA
UniProt:
P54920 (Human)