ProSci

ACTR3 Antibody

Product Code:
 
PSI-13-766
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using ACTR3 antibody (13-766) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
2 / 2
Immunofluorescence analysis of HeLa cells using ACTR3 antibody (13-766). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ACTR3 antibody (13-766) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunofluorescence analysis of HeLa cells using ACTR3 antibody (13-766). Blue: DAPI for nuclear staining.

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PSI-13-766-50uL50uL£433.00
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Further Information

Additional Names:
Actin-related protein 3, Actin-like protein 3, ACTR3, ARP3
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-418 of human ACTR3 (NP_005712.1).
NCBI Gene ID #:
10096
NCBI Official Name:
Actin-related protein 3
NCBI Official Symbol:
ACTR3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Innate Immunity
Swissprot #:
P61158
User NOte:
Optimal dilutions for each application to be determined by the researcher.