ProSci

HSD17B1 Antibody

Product Code:
 
PSI-13-829
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using HSD17B1 antibody (13-829) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 5
Immunohistochemistry of paraffin-embedded human placenta using HSD17B1 antibody (13-829) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded mouse heart using HSD17B1 antibody (13-829) at dilution of 1:100 (40x lens).
4 / 5
Immunofluorescence analysis of C6 cells using HSD17B1 Polyclonal Antibody (13-829) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
5 / 5
Immunofluorescence analysis of HeLa cells using HSD17B1 Polyclonal Antibody (13-829) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using HSD17B1 antibody (13-829) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human placenta using HSD17B1 antibody (13-829) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HSD17B1 antibody (13-829) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using HSD17B1 Polyclonal Antibody (13-829) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using HSD17B1 Polyclonal Antibody (13-829) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-13-829-50uL50uL£433.00
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Further Information

Additional Names:
HSD17B1, EDH17B2, EDHB17, HSD17, SDR28C1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human HSD17B1 (NP_000404.2).
NCBI Gene ID #:
3292
NCBI Official Name:
hydroxysteroid (17-beta) dehydrogenase 1
NCBI Official Symbol:
HSD17B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Neuroscience, Signal Transduction
Swissprot #:
P14061
User NOte:
Optimal dilutions for each application to be determined by the researcher.