ProSci

PHB Antibody

Product Code:
 
PSI-15-044
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using PHB antibody (15-044) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunofluorescence analysis of HeLa cells using PHB antibody (15-044). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PHB antibody (15-044) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using PHB antibody (15-044). Blue: DAPI for nuclear staining.

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PSI-15-044-50uL50uL£433.00
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Further Information

Additional Names:
PHB, PHB1, HEL-215, HEL-S-54e
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 73-272 of human PHB (NP_002625.1).
NCBI Gene ID #:
5245
NCBI Official Name:
prohibitin
NCBI Official Symbol:
PHB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
P35232
User NOte:
Optimal dilutions for each application to be determined by the researcher.