ProSci

BCHE Antibody

Product Code:
 
PSI-15-622
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using BCHE antibody (15-622) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 3
Immunohistochemistry of paraffin-embedded rat kidney using BCHE antibody (15-622) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of U2OS cells using BCHE antibody (15-622). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using BCHE antibody (15-622) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using BCHE antibody (15-622) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using BCHE antibody (15-622). Blue: DAPI for nuclear staining.

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PSI-15-622-50uL50uL£433.00
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Further Information

Additional Names:
BCHE, CHE1, E1, CHE2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 29-270 of human BCHE (NP_000046.1).
NCBI Gene ID #:
590
NCBI Official Name:
butyrylcholinesterase
NCBI Official Symbol:
BCHE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P06276
User NOte:
Optimal dilutions for each application to be determined by the researcher.