ProSci

GFAP Antibody

Product Code:
 
PSI-15-662
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using GFAP antibody (15-662) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using GFAP antibody (15-662) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP antibody (15-662) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using GFAP antibody (15-662) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U-251MG cells using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of rat brain using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of mouse brain using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GFAP antibody (15-662) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using GFAP antibody (15-662) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP antibody (15-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GFAP antibody (15-662) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using GFAP antibody (15-662) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-15-662-50uL50uL£433.00
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Further Information

Additional Names:
Glial fibrillary acidic protein, GFAP, GFAP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).
NCBI Gene ID #:
2670
NCBI Official Name:
glial fibrillary acidic protein
NCBI Official Symbol:
GFAP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P14136
User NOte:
Optimal dilutions for each application to be determined by the researcher.