ProSci

SLC12A5 Antibody

Product Code:
 
PSI-16-068
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of mouse brain, using SLC12A5 antibody (16-068) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Western blot analysis of extracts of mouse brain, using SLC12A5 antibody (16-068) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.

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PSI-16-068-50uL50uL£433.00
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Further Information

Additional Names:
EIEE34, EIG14, KCC2, hKCC2, solute carrier family 12 member 5, K-Cl cotransporter 2, electroneutral potassium-chloride cotransporter 2, erythroid K-Cl cotransporter 2, neuronal K-Cl cotransporter, solute carrier family 12 (potassium/chloride transporter), member 5
Application Note:
WB: 1:200 - 1:2000
Background:
K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 945-1025 of human SLC12A5 (NP_001128243.1).
NCBI Gene ID #:
57468
NCBI Official Name:
solute carrier family 12 member 5
NCBI Official Symbol:
SLC12A5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 135kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q9H2X9
User NOte:
Optimal dilutions for each application to be determined by the researcher.