ProSci

SPARC Antibody

Product Code:
 
PSI-16-455
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using SPARC antibody (16-455) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 4
Immunohistochemistry of paraffin-embedded rat Intestine using SPARC antibody (16-455) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human lung cancer using SPARC antibody (16-455) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse kidney using SPARC antibody (16-455) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using SPARC antibody (16-455) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat Intestine using SPARC antibody (16-455) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SPARC antibody (16-455) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SPARC antibody (16-455) at dilution of 1:100 (40x lens).

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PSI-16-455-50uL50uL£433.00
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Further Information

Additional Names:
SPARC, Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, SPARC, ON
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1).
NCBI Gene ID #:
6678
NCBI Official Name:
SPARC
NCBI Official Symbol:
SPARC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P09486
User NOte:
Optimal dilutions for each application to be determined by the researcher.