ProSci

SON Antibody

Product Code:
 
PSI-16-553
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 1
Western blot analysis of extracts of various cell lines, using SON antibody (16-553) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 8min.
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Western blot analysis of extracts of various cell lines, using SON antibody (16-553) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 8min.

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PSI-16-553-50uL50uL£433.00
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Further Information

Additional Names:
negative regulatory element-binding protein, OTTHUMP00000198934, OTTHUMP00000197908, NRE-binding protein, Bax antagonist selected in Saccharomyces 1, NREBP, KIAA1019, FLJ33914, FLJ21099, DBP-5, C21orf50, BASS1, SON DNA binding protein, SON, DBP5, HSPC310, HSPC312
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a protein that contains multiple simple repeats. The encoded protein binds RNA and promotes pre-mRNA splicing, particularly of transcripts with poor splice sites. The protein also recognizes a specific DNA sequence found in the human hepatitis B virus (HBV) and represses HBV core promoter activity. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 850-950 of human SON (NP_001278340.1).
NCBI Gene ID #:
6651
NCBI Official Name:
SON DNA binding protein
NCBI Official Symbol:
SON
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 250kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle
Swissprot #:
P18583
User NOte:
Optimal dilutions for each application to be determined by the researcher.