ProSci

Lamin B1 Antibody

Product Code:
 
PSI-16-793
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (16-793) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human breast using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human tonsil using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (16-793) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (16-793) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (16-793) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-16-793-50uL50uL£433.00
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Further Information

Additional Names:
LMNB1, lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419, OTTHUMP00000159218, lamin-B1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human Lamin B1
NCBI Gene ID #:
4001
NCBI Official Name:
lamin B1
NCBI Official Symbol:
LMNB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 75kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction
Swissprot #:
P20700
User NOte:
Optimal dilutions for each application to be determined by the researcher.