ProSci

BUB1B Antibody

Product Code:
 
PSI-18-086
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using BUB1B antibody (18-086) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunohistochemistry of paraffin-embedded human breast cancer using BUB1B antibody (18-086) at dilution of 1:200 (40x lens).
3 / 3
Immunofluorescence analysis of NIH-3T3 cells using BUB1B antibody (18-086) at dilution of 1:100. Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using BUB1B antibody (18-086) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast cancer using BUB1B antibody (18-086) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using BUB1B antibody (18-086) at dilution of 1:100. Blue: DAPI for nuclear staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-18-086-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
BUB1B, Bub1A, BUBR1, HBUBR1, Protein SSK1, SSK1, BUB1beta, MAD3L
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BUB1B (NP_001202.4).
NCBI Gene ID #:
701
NCBI Official Name:
BUB1 mitotic checkpoint serine/threonine kinase B
NCBI Official Symbol:
BUB1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa/120kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Signal Transduction
Swissprot #:
O60566
User NOte:
Optimal dilutions for each application to be determined by the researcher.