ProSci

PPA1 Antibody

Product Code:
 
PSI-18-507
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using PPA1 antibody (18-507) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunohistochemistry of paraffin-embedded rat brain using PPA1 Antibody (18-507) at dilution of 1:200 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded rat heart using PPA1 Antibody (18-507) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using PPA1 antibody (18-507) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using PPA1 Antibody (18-507) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using PPA1 Antibody (18-507) at dilution of 1:200 (40x lens).

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PSI-18-507-50uL50uL£433.00
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Further Information

Additional Names:
PPA1, IOPPP, MGC111556, PP, PP1, SID6-8061, HEL-S-66p
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PPA1 (NP_066952.1).
NCBI Gene ID #:
5464
NCBI Official Name:
pyrophosphatase (inorganic) 1
NCBI Official Symbol:
PPA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q15181
User NOte:
Optimal dilutions for each application to be determined by the researcher.