ProSci

CCNG1 Antibody

Product Code:
 
PSI-19-565
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using CCNG1 antibody (19-565) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 2
Immunofluorescence analysis of 293T cells using CCNG1 antibody (19-565) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CCNG1 antibody (19-565) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of 293T cells using CCNG1 antibody (19-565) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-19-565-50uL50uL£433.00
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Further Information

Additional Names:
Cyclin-G1, Cyclin-G, CCNG1, CCNG, CYCG1
Application Note:
WB: 1:500 - 1:1000

IF: 1:20 - 1:100
Background:
The eukaryotic cell cycle is governed by cyclin-dependent protein kinases (CDKs) whose activities are regulated by cyclins and CDK inhibitors. The protein encoded by this gene is a member of the cyclin family and contains the cyclin box. The encoded protein lacks the protein destabilizing (PEST) sequence that is present in other family members. Transcriptional activation of this gene can be induced by tumor protein p53. Two transcript variants encoding the same protein have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human CCNG1 (NP_004051.1).
NCBI Gene ID #:
900
NCBI Official Name:
Cyclin-G1
NCBI Official Symbol:
CCNG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 34kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P51959
User NOte:
Optimal dilutions for each application to be determined by the researcher.