ProSci

EIF4A1 Antibody

Product Code:
 
PSI-19-567
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using EIF4A1 antibody (19-567) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 3
Immunofluorescence analysis of MCF-7 cells using EIF4A1 antibody (19-567). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells using 3ug EIF4A1 antibody (19-567). Western blot was performed from the immunoprecipitate using EIF4A1 antibody (19-567) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using EIF4A1 antibody (19-567) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of MCF-7 cells using EIF4A1 antibody (19-567). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells using 3ug EIF4A1 antibody (19-567). Western blot was performed from the immunoprecipitate using EIF4A1 antibody (19-567) at a dilition of 1:1000.

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PSI-19-567-50uL50uL£433.00
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Further Information

Additional Names:
DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I, eukaryotic initiation factor 4A-I, ATP-dependent RNA helicase eIF4A-1, eukaryotic initiation factor 4AI, eukaryotic translation initiation factor 4A
Application Note:
WB: 1:500 - 1:2000

IF: 1:20 - 1:50

IP: 1:20 - 1:50
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-406 of human EIF4A1 (NP_001407.1).
NCBI Gene ID #:
1973
NCBI Official Name:
eukaryotic translation initiation factor 4A1
NCBI Official Symbol:
EIF4A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Immunology
Swissprot #:
P60842
User NOte:
Optimal dilutions for each application to be determined by the researcher.