ProSci

CPT1A Antibody

Product Code:
 
PSI-19-575
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of 22Rv1 cells, using CPT1A antibody (19-575) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5min.
2 / 2
Immunofluorescence analysis of A-549 cells using CPT1A antibody (19-575). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of 22Rv1 cells, using CPT1A antibody (19-575) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5min.
Immunofluorescence analysis of A-549 cells using CPT1A antibody (19-575). Blue: DAPI for nuclear staining.

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PSI-19-575-50uL50uL£433.00
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Further Information

Additional Names:
CPT1A, CPT1, CPT1-L, L-CPT1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 497-756 of human CPT1A (NP_001027017.1).
NCBI Gene ID #:
1374
NCBI Official Name:
carnitine palmitoyltransferase 1A (liver)
NCBI Official Symbol:
CPT1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 90kDa
Purification:
Affinity purification
Research Area:
Cancer, Obesity, Signal Transduction
Swissprot #:
P50416
User NOte:
Optimal dilutions for each application to be determined by the researcher.