ProSci

APTX Antibody

Product Code:
 
PSI-19-618
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using APTX antibody (19-618) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunofluorescence analysis of A549 cells using APTX antibody (19-618).
3 / 3
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody (19-618). Western blot was performed from the immunoprecipitate using APTX antibody (19-618) at a dilition of 1:500.
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Western blot analysis of extracts of various cell lines, using APTX antibody (19-618) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using APTX antibody (19-618).
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody (19-618). Western blot was performed from the immunoprecipitate using APTX antibody (19-618) at a dilition of 1:500.

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PSI-19-618-50uL50uL£433.00
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Further Information

Additional Names:
APTX, AOA, AOA1, AXA1, EAOH, EOAHA, FHA-HIT
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100

IP: 1:20 - 1:50
Background:
This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1).
NCBI Gene ID #:
54840
NCBI Official Name:
aprataxin
NCBI Official Symbol:
APTX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q7Z2E3
User NOte:
Optimal dilutions for each application to be determined by the researcher.